cfvr 488a goat anti mouse secondary antibody Search Results


donkey anti mouse cf 488a  (Biotium)
95
Biotium donkey anti mouse cf 488a
Donkey Anti Mouse Cf 488a, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cf 488a  (Biotium)
94
Biotium cf 488a
Cf 488a, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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rabbit anti map4  (Fortis Life Sciences)
93
Fortis Life Sciences rabbit anti map4
A, <t>MAP4</t> KD impairs p110α distribution along microtubules. MDA-MB-231 cells were transfected with control siRNAs, p85α siRNAs or combination of p85α and MAP4 siRNAs. 48-72 hours post-transfection, cells were fixed with methanol. The co-localization of p110α catalytic subunit with microtubules was examined by IF using antibodies specific to p110α (red) and beta-tubulin (green). B, increased association of p110α with MAP4 upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or p85α siRNAs. 48-72 hours post-transfection, cells were harvested and endogenous p110α IPed and the MAP4 co-IPed with p110α was examined by WB. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl versus sip85α, EGF stimulated). C, examination of p110α association with MAP4 by PLA. MDA-MB-231 cells were transfected with control siRNAs or siRNA stargeting p85α. 48-72 hours post-transfection, cells were fixed with 4% PFA and processed for PLA. The PLA foci were counted from at least 20 cells. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl vs sip85α, EGF stimulated). Related to Main
Rabbit Anti Map4, supplied by Fortis Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfvr+488a+goat+anti+mouse+secondary+antibody/MAP4+Antibody/bio_rxiv__2024__01__10__575035-116-72-75
Average 93 stars, based on 1 article reviews
rabbit anti map4 - by Bioz Stars, 2026-10
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pbs  (Thermo Fisher)
99
Thermo Fisher pbs
A, <t>MAP4</t> KD impairs p110α distribution along microtubules. MDA-MB-231 cells were transfected with control siRNAs, p85α siRNAs or combination of p85α and MAP4 siRNAs. 48-72 hours post-transfection, cells were fixed with methanol. The co-localization of p110α catalytic subunit with microtubules was examined by IF using antibodies specific to p110α (red) and beta-tubulin (green). B, increased association of p110α with MAP4 upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or p85α siRNAs. 48-72 hours post-transfection, cells were harvested and endogenous p110α IPed and the MAP4 co-IPed with p110α was examined by WB. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl versus sip85α, EGF stimulated). C, examination of p110α association with MAP4 by PLA. MDA-MB-231 cells were transfected with control siRNAs or siRNA stargeting p85α. 48-72 hours post-transfection, cells were fixed with 4% PFA and processed for PLA. The PLA foci were counted from at least 20 cells. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl vs sip85α, EGF stimulated). Related to Main
Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cfvr+488a+goat+anti+mouse+secondary+antibody/PBS/pmc04594298-300-27-59
Average 99 stars, based on 1 article reviews
pbs - by Bioz Stars, 2026-10
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a300 488a rrid ab 2175262  (Bethyl)
93
Bethyl a300 488a rrid ab 2175262
KEY RESOURCES TABLE
A300 488a Rrid Ab 2175262, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti guinea pig igg polyclonal cf 488a  (Biotium)
92
Biotium goat anti guinea pig igg polyclonal cf 488a
KEY RESOURCES TABLE
Goat Anti Guinea Pig Igg Polyclonal Cf 488a, supplied by Biotium, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti mouse igg2a alexa fluor 488  (Proteintech)
96
Proteintech goat anti mouse igg2a alexa fluor 488
KEY RESOURCES TABLE
Goat Anti Mouse Igg2a Alexa Fluor 488, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cf tm 488a goat anti rabbit igg h l  (Biotium)
95
Biotium cf tm 488a goat anti rabbit igg h l
KEY RESOURCES TABLE
Cf Tm 488a Goat Anti Rabbit Igg H L, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rabbit alexa fluor 488  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology goat anti rabbit alexa fluor 488
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Goat Anti Rabbit Alexa Fluor 488, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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donkey anti mouse alexa fluortm 488  (Fisher Scientific)
86
Fisher Scientific donkey anti mouse alexa fluortm 488
KEY RESOURCES TABLE
Donkey Anti Mouse Alexa Fluortm 488, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse secondary antibody  (Biotium)
99
Biotium anti mouse secondary antibody
KEY RESOURCES TABLE
Anti Mouse Secondary Antibody, supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse secondary antibody - by Bioz Stars, 2026-10
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goat anti mouse  (Jackson Immuno)
93
Jackson Immuno goat anti mouse
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Goat Anti Mouse, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A, MAP4 KD impairs p110α distribution along microtubules. MDA-MB-231 cells were transfected with control siRNAs, p85α siRNAs or combination of p85α and MAP4 siRNAs. 48-72 hours post-transfection, cells were fixed with methanol. The co-localization of p110α catalytic subunit with microtubules was examined by IF using antibodies specific to p110α (red) and beta-tubulin (green). B, increased association of p110α with MAP4 upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or p85α siRNAs. 48-72 hours post-transfection, cells were harvested and endogenous p110α IPed and the MAP4 co-IPed with p110α was examined by WB. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl versus sip85α, EGF stimulated). C, examination of p110α association with MAP4 by PLA. MDA-MB-231 cells were transfected with control siRNAs or siRNA stargeting p85α. 48-72 hours post-transfection, cells were fixed with 4% PFA and processed for PLA. The PLA foci were counted from at least 20 cells. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl vs sip85α, EGF stimulated). Related to Main

Journal:

Article Title:

doi:

Figure Lengend Snippet: A, MAP4 KD impairs p110α distribution along microtubules. MDA-MB-231 cells were transfected with control siRNAs, p85α siRNAs or combination of p85α and MAP4 siRNAs. 48-72 hours post-transfection, cells were fixed with methanol. The co-localization of p110α catalytic subunit with microtubules was examined by IF using antibodies specific to p110α (red) and beta-tubulin (green). B, increased association of p110α with MAP4 upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or p85α siRNAs. 48-72 hours post-transfection, cells were harvested and endogenous p110α IPed and the MAP4 co-IPed with p110α was examined by WB. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl versus sip85α, EGF stimulated). C, examination of p110α association with MAP4 by PLA. MDA-MB-231 cells were transfected with control siRNAs or siRNA stargeting p85α. 48-72 hours post-transfection, cells were fixed with 4% PFA and processed for PLA. The PLA foci were counted from at least 20 cells. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siControl vs sip85α, EGF stimulated). Related to Main

Article Snippet: Rabbit anti-p110α (#4249, Cell Signaling), rabbit anti-p110α (#ab40776, Abcam), rabbit anti-p110β (#3011, Cell Signaling), rabbit anti-p85α (#ab191606, Abcam), mouse anti-p85β (#MAB6777, R and D), mouse anti-PI3,4,5P 3 antibody (Z-P345, Echelon Biosciences), rabbit anti-Akt (#ab126811, Abcam), rabbit anti-Akt-HRP (#4298, Cell Signaling), rabbit anti-phospho-Akt T308 (#2965, Cell Signaling), rabbit anti-phospho-Akt S473 (#4060, Cell Signaling), rabbit anti-pAkt (OMA1-03061, Life Technologies), rabbit anti-phospho Akt (#OMA1-03061, Invitrogen), mouse anti-phospho Akt (#200-301-268S, Rockland), mouse anti-MAP4 (#sc-390286, Santa Cruz), rabbit anti-MAP4 (#A301-488A, Bethyl), mouse anti-MAP4 agarose beads (#sc-390286 AC, Santa Cruz), normal mouse IgG beads (sc-2343, Santa Cruz), rabbit anti-HA (#3724, Cell Signaling), mouse anti-HA agarose beads (#26181, ThermoFisher), anti-GST HRP conjugate (#RPN 1236V, Amersham), rabbit anti-Flag (#2368, Cell Signaling), mouse anti-Flag (#F1804, Sigma), anti-Flag M2 affinity gel (#F2426, Sigma-Aldrich), rabbit anti-GAPDH (#2118, Cell Signaling), rabbit anti-tubulin (#ab18251, Abcam), mouse anti-EEA1 (#610457, BD Biosciences), rabbit anti-EEA1 (#3288, Cell Signaling), mouse anti-TFR (#136800, Invitrogen), rabbit anti-TFR (#ab84036, Abcam), rat anti-tubulin (#ab6160, Abcam), rabbit anti-tubulin (#ab6046, Abcam), normal rabbit IgG beads (#2729, Cell Signaling), rabbit anti-EGFR beads (#5735 Cell Signaling), rabbit anti-EGFR (sc-03, Santa Cruz), mouse anti-EGFR (#sc-120, Santa Cruz), anti-beta actin (#4967), rabbit anti-Na+/K+ ATPase (#3010, Cell Signaling) and PTEN (sc-7974, Santa Cruz).

Techniques: Transfection

A, p110α localizes along microtubules and associates with MAP4 upon p85α KD. Upper panel, MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were fixed with 4% PFA. The distribution of p110α along microtubules in cells treated with control and p85α siRNAs was examined by IF using antibodies specific for p110α (red) and MAP4 (green). Similarly, the agonist-stimulated association of residual p110α with MAP4 upon p85α KD by co-IP. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siCon versus p85α siRNA). B, C, p110α co-localizes with endosomal markers upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or siRNA targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF and fixed with 4% PFA. The co-localization of p110α with endosomal markers in cells treated with control and p85α siRNAs was examined by IF using antibodies specific for p110α (red) and endosomes (EEA1 and TFR) (green). The number of EEA1- and TFR-puncta positive for p110α was counted in at least 20-30 cells. The statistical significance is indicated by p-value (control versus p85α KD, EGF stimulated). D, EGF-stimulated p110α association with EGFR upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF before harvesting the cells. EGFR was IPed using anti-EGFR antibody beads, and co-IPed p110α examined by WB. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siCon versus p85α siRNA, EGF stimulated). E, EGF-stimulated p110α co-localization with EGFR upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF for 5 minutes before fixation with 4% PFA. The co-localization of p110α with EGFR was examined by IF study using antibodies specific for p110α (red) and EGFR (green). EGFR-puncta positive for p110α were counted in at least 20-30 cells. The statistical significance is indicated by p-value (control versus p85α KD, EGF stimulated). F, EGF-stimulated p110α association with EGFR upon p85α KD by PLA. MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF for 5 minutes before fixation with 4% PFA. Cells were incubated with p110α and EGFR-specific primary antibodies followed by PLA assay. PLA puncta were counted in at least 20-30 cells. The statistical significance is indicated by the p-value (control versus p85α KD, EGF stimulated).

Journal:

Article Title:

doi:

Figure Lengend Snippet: A, p110α localizes along microtubules and associates with MAP4 upon p85α KD. Upper panel, MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were fixed with 4% PFA. The distribution of p110α along microtubules in cells treated with control and p85α siRNAs was examined by IF using antibodies specific for p110α (red) and MAP4 (green). Similarly, the agonist-stimulated association of residual p110α with MAP4 upon p85α KD by co-IP. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siCon versus p85α siRNA). B, C, p110α co-localizes with endosomal markers upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or siRNA targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF and fixed with 4% PFA. The co-localization of p110α with endosomal markers in cells treated with control and p85α siRNAs was examined by IF using antibodies specific for p110α (red) and endosomes (EEA1 and TFR) (green). The number of EEA1- and TFR-puncta positive for p110α was counted in at least 20-30 cells. The statistical significance is indicated by p-value (control versus p85α KD, EGF stimulated). D, EGF-stimulated p110α association with EGFR upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF before harvesting the cells. EGFR was IPed using anti-EGFR antibody beads, and co-IPed p110α examined by WB. The data represent the mean ± SD from three independent experiments. The statistical significance is indicated by p-value (siCon versus p85α siRNA, EGF stimulated). E, EGF-stimulated p110α co-localization with EGFR upon p85α KD. MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF for 5 minutes before fixation with 4% PFA. The co-localization of p110α with EGFR was examined by IF study using antibodies specific for p110α (red) and EGFR (green). EGFR-puncta positive for p110α were counted in at least 20-30 cells. The statistical significance is indicated by p-value (control versus p85α KD, EGF stimulated). F, EGF-stimulated p110α association with EGFR upon p85α KD by PLA. MDA-MB-231 cells were transfected with control siRNAs or siRNAs targeting p85α. 48-72 hours post-transfection, cells were stimulated with EGF for 5 minutes before fixation with 4% PFA. Cells were incubated with p110α and EGFR-specific primary antibodies followed by PLA assay. PLA puncta were counted in at least 20-30 cells. The statistical significance is indicated by the p-value (control versus p85α KD, EGF stimulated).

Article Snippet: Rabbit anti-p110α (#4249, Cell Signaling), rabbit anti-p110α (#ab40776, Abcam), rabbit anti-p110β (#3011, Cell Signaling), rabbit anti-p85α (#ab191606, Abcam), mouse anti-p85β (#MAB6777, R and D), mouse anti-PI3,4,5P 3 antibody (Z-P345, Echelon Biosciences), rabbit anti-Akt (#ab126811, Abcam), rabbit anti-Akt-HRP (#4298, Cell Signaling), rabbit anti-phospho-Akt T308 (#2965, Cell Signaling), rabbit anti-phospho-Akt S473 (#4060, Cell Signaling), rabbit anti-pAkt (OMA1-03061, Life Technologies), rabbit anti-phospho Akt (#OMA1-03061, Invitrogen), mouse anti-phospho Akt (#200-301-268S, Rockland), mouse anti-MAP4 (#sc-390286, Santa Cruz), rabbit anti-MAP4 (#A301-488A, Bethyl), mouse anti-MAP4 agarose beads (#sc-390286 AC, Santa Cruz), normal mouse IgG beads (sc-2343, Santa Cruz), rabbit anti-HA (#3724, Cell Signaling), mouse anti-HA agarose beads (#26181, ThermoFisher), anti-GST HRP conjugate (#RPN 1236V, Amersham), rabbit anti-Flag (#2368, Cell Signaling), mouse anti-Flag (#F1804, Sigma), anti-Flag M2 affinity gel (#F2426, Sigma-Aldrich), rabbit anti-GAPDH (#2118, Cell Signaling), rabbit anti-tubulin (#ab18251, Abcam), mouse anti-EEA1 (#610457, BD Biosciences), rabbit anti-EEA1 (#3288, Cell Signaling), mouse anti-TFR (#136800, Invitrogen), rabbit anti-TFR (#ab84036, Abcam), rat anti-tubulin (#ab6160, Abcam), rabbit anti-tubulin (#ab6046, Abcam), normal rabbit IgG beads (#2729, Cell Signaling), rabbit anti-EGFR beads (#5735 Cell Signaling), rabbit anti-EGFR (sc-03, Santa Cruz), mouse anti-EGFR (#sc-120, Santa Cruz), anti-beta actin (#4967), rabbit anti-Na+/K+ ATPase (#3010, Cell Signaling) and PTEN (sc-7974, Santa Cruz).

Techniques: Transfection, Co-Immunoprecipitation Assay, Incubation

A, MDA-MB-231 cells stably expressing HA-tagged WT p110α or 4Q mutant p110α were stimulated with EGF for 5 minutes before harvesting the cells. p110α was IPed using anti-HA antibody agarose beads and the co-IPed endogenous EGFR was examined by WB. The data is representative of three independent experiments. B, C, MDA-MB-231 cells stably expressing HA-tagged WT or 4Q mutant p110α were stimulated with EGF for 3-5 minutes before fixing the cells with 4% PFA. The cells were immuno-stained with antibodies for HA (red) and EGFR (green). The EGFR-puncta that co-localized with HA were counted in least 20-30 cells. The statistical significance is indicated by p-value (p110α WT versus p110α 4Q, EGF stimulated). D, COS-7 cells transiently expressing HA-tagged WT p110α or 4Q mutant p110α were stimulated with EGF for 5 minutes before harvesting the cells. Endogenous EGFR was IPed and the co-IPed p110α was examined by WB. The data is representative of three independent experiments. E, F, COS-7 cells transiently expressing HA-tagged WT or 4Q mutant p110α were stimulated with EGF for 3-5 minutes before fixing the cells with 4% PFA. The cells were processed for PLA assay. The PLA punta were counted in least 20-30 cells and normalized to control cells. The statistical significance is indicated by p-value (p110α WT versus p110α 4Q, EGF stimulated). G, Schematic diagram depicting the mechanism on how loss of p85α adaptor subunit promotes agonist stimulated PI3K/Akt signaling in the endomembrane. The loss of p85α adaptor subunit promotes the spatial localization of residual p110α catalytic subunit along microtubules via MAP4 binding and integration into endosomal membranes via p110α C2 domain and endosomal PI3P interaction. This leads to increased interaction of residual p110α catalytic subunit with activated endosomal receptor, promoting PI3K/Akt signaling in the endosomal membrane.

Journal:

Article Title:

doi:

Figure Lengend Snippet: A, MDA-MB-231 cells stably expressing HA-tagged WT p110α or 4Q mutant p110α were stimulated with EGF for 5 minutes before harvesting the cells. p110α was IPed using anti-HA antibody agarose beads and the co-IPed endogenous EGFR was examined by WB. The data is representative of three independent experiments. B, C, MDA-MB-231 cells stably expressing HA-tagged WT or 4Q mutant p110α were stimulated with EGF for 3-5 minutes before fixing the cells with 4% PFA. The cells were immuno-stained with antibodies for HA (red) and EGFR (green). The EGFR-puncta that co-localized with HA were counted in least 20-30 cells. The statistical significance is indicated by p-value (p110α WT versus p110α 4Q, EGF stimulated). D, COS-7 cells transiently expressing HA-tagged WT p110α or 4Q mutant p110α were stimulated with EGF for 5 minutes before harvesting the cells. Endogenous EGFR was IPed and the co-IPed p110α was examined by WB. The data is representative of three independent experiments. E, F, COS-7 cells transiently expressing HA-tagged WT or 4Q mutant p110α were stimulated with EGF for 3-5 minutes before fixing the cells with 4% PFA. The cells were processed for PLA assay. The PLA punta were counted in least 20-30 cells and normalized to control cells. The statistical significance is indicated by p-value (p110α WT versus p110α 4Q, EGF stimulated). G, Schematic diagram depicting the mechanism on how loss of p85α adaptor subunit promotes agonist stimulated PI3K/Akt signaling in the endomembrane. The loss of p85α adaptor subunit promotes the spatial localization of residual p110α catalytic subunit along microtubules via MAP4 binding and integration into endosomal membranes via p110α C2 domain and endosomal PI3P interaction. This leads to increased interaction of residual p110α catalytic subunit with activated endosomal receptor, promoting PI3K/Akt signaling in the endosomal membrane.

Article Snippet: Rabbit anti-p110α (#4249, Cell Signaling), rabbit anti-p110α (#ab40776, Abcam), rabbit anti-p110β (#3011, Cell Signaling), rabbit anti-p85α (#ab191606, Abcam), mouse anti-p85β (#MAB6777, R and D), mouse anti-PI3,4,5P 3 antibody (Z-P345, Echelon Biosciences), rabbit anti-Akt (#ab126811, Abcam), rabbit anti-Akt-HRP (#4298, Cell Signaling), rabbit anti-phospho-Akt T308 (#2965, Cell Signaling), rabbit anti-phospho-Akt S473 (#4060, Cell Signaling), rabbit anti-pAkt (OMA1-03061, Life Technologies), rabbit anti-phospho Akt (#OMA1-03061, Invitrogen), mouse anti-phospho Akt (#200-301-268S, Rockland), mouse anti-MAP4 (#sc-390286, Santa Cruz), rabbit anti-MAP4 (#A301-488A, Bethyl), mouse anti-MAP4 agarose beads (#sc-390286 AC, Santa Cruz), normal mouse IgG beads (sc-2343, Santa Cruz), rabbit anti-HA (#3724, Cell Signaling), mouse anti-HA agarose beads (#26181, ThermoFisher), anti-GST HRP conjugate (#RPN 1236V, Amersham), rabbit anti-Flag (#2368, Cell Signaling), mouse anti-Flag (#F1804, Sigma), anti-Flag M2 affinity gel (#F2426, Sigma-Aldrich), rabbit anti-GAPDH (#2118, Cell Signaling), rabbit anti-tubulin (#ab18251, Abcam), mouse anti-EEA1 (#610457, BD Biosciences), rabbit anti-EEA1 (#3288, Cell Signaling), mouse anti-TFR (#136800, Invitrogen), rabbit anti-TFR (#ab84036, Abcam), rat anti-tubulin (#ab6160, Abcam), rabbit anti-tubulin (#ab6046, Abcam), normal rabbit IgG beads (#2729, Cell Signaling), rabbit anti-EGFR beads (#5735 Cell Signaling), rabbit anti-EGFR (sc-03, Santa Cruz), mouse anti-EGFR (#sc-120, Santa Cruz), anti-beta actin (#4967), rabbit anti-Na+/K+ ATPase (#3010, Cell Signaling) and PTEN (sc-7974, Santa Cruz).

Techniques: Stable Transfection, Expressing, Mutagenesis, Staining, Binding Assay, Membrane

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance

doi: 10.1016/j.celrep.2018.08.086

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit Anti-CtIP Polyclonal antibody , Bethyl , Cat# A300–488A; RRID: AB_2175262.

Techniques: Labeling, Polymer, Recombinant, Plasmid Preparation, Staining, Mutagenesis, Extraction, Expressing, TaqMan Assay, shRNA, Software